Clarification about streaking of western membranes

Demeke, Tigst tdemeke at
Tue Nov 19 09:38:34 EST 1996

I was asked about the type and percentage of gel I was using.  I use either 
10% homogeneous or 8-12% gradient gel (SDS-PAGE).  My question was how do 
you I get rid off streaks that consistently appear on nitrocellulose and 
PVDF membranes.

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