histag_experiment

Torben Gjetting tor at kennedy.dk
Tue Nov 19 03:36:36 EST 1996


I am currently planning an experiment which allows me to purify
my histagged protein (ca. 60kDa). 
I have purchased the Ni-NTA agarose resin
from Qiagen, and I am thinking about using the Bio-Spin columns from
BIO-RAD.
The reason for using spin columns is that I would like to be able to 
do a series of purifications in parallel. If a better method that I 
am not aware of is available, I will be most grateful for the advise.
In addition, I would greatly apppreciate if someone can recommend an
outline of the
protocol for the purification. 

Thank you in advance.
Torben Gjetting

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