Protein binding to eppendorf/cuvette walls.
ipark at bioneer.kaist.ac.kr
Wed Nov 20 12:14:18 EST 1996
qi bao (q-bao at nimr.mrc.ac.uk) wrote:
: I am working with a protein that seems to bind strongly to the
: walls of eppendorfs and cuvettes.
: If I put a certain concentration of protein into one of these vessels
: then remove it, the concentration is reduced considerably, (no
: precipitation observed).
: Has anyone had any experience with this kind of problem?
: Anyone got any good ideas how I can get around this?
: The data that I am trying to get does not allow me to try using
: other non-specific proteins in the vessels.
: Also, I know that siliconising the walls may be a solution but
: presumeably when doing this the extent of siliconisation from tube
: to tube will vary and may affect the reproduciblity of results.
: Any ideas will be gratefully received (e-mail or newsgroup or both)
: Dave (d-hollin at nimr.mrc.ac.uk)
How about vortexing the hell out of it? :)
(It worked for me... Just stick it into the vortexer and go to lunch,
dinner, or whatever, and when you come back... voila!)
Korea Advanced Institute
of Science and Technology
U Penn '95
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