coupling proteins to CNBr activated sepharose...
Dr. David Martin
david.martin at biotek.uio.no
Wed Nov 20 05:07:10 EST 1996
In article <siyer-1911961421520001 at bmg147.cmc.uab.edu>,
siyer at bmg.bhs.uab.edu (Sai) wrote:
> anybody here try to couple really diluted protein samples to the
>CNBr activated sepharose 4B resin ? what i mean is that the protocol by
>pharmacia recommneds that the coupling solution be really concentrated
>(about 5 mg or protein in a 1-2 ml coupling reaction) and this may not be
>too feasible for some of us (i.e me) who have proteins that dont like
>being too crowded (aggregration in hi concs.). so i was thinking to
>dilute out my coupling reaction with 10 mg or protein in a 30 - 40 ml
>solution. that way, i dont have to worry about aggregation problems
>during the coupling reaction. so would dilution to that much of an extent
>affect coupling efficiency?? any ideas? thanks...
It should go fine.. just make sure you agitate well.
* David Martin - Atherosclerosis and Thrombosis research group *
* http://www.uio.no/~damartin/ david.martin at biotek.uio.no *
More information about the Proteins