coupling proteins to CNBr activated sepharose...

Dr. David Martin david.martin at
Wed Nov 20 05:07:10 EST 1996

In article <siyer-1911961421520001 at>,
   siyer at (Sai) wrote:
>hey all,
>        anybody here try to couple really diluted protein samples to the
>CNBr activated sepharose 4B resin ?  what i mean is that the protocol by
>pharmacia recommneds that the coupling solution be really concentrated
>(about 5 mg or protein in a 1-2 ml coupling reaction) and this may not be
>too feasible for some of us (i.e me) who have proteins that dont like
>being too crowded (aggregration in hi concs.).  so i was thinking to
>dilute out my coupling reaction with 10 mg or protein in a 30 - 40 ml
>solution.  that way, i dont have to worry about aggregation problems
>during the coupling reaction.  so would dilution to that much of an extent
>affect coupling efficiency??  any ideas?  thanks...

It should go fine.. just make sure you agitate well.


* David Martin - Atherosclerosis and Thrombosis research group  *
*      david.martin at  *

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