Why freeze autorads?
Dr. Peter Gegenheimer
PGegen at RNAWorld.bio.UKans.edu
Sat Nov 23 03:27:08 EST 1996
In <57008p$1nem at news.doit.wisc.edu>, klenchin at facstaff.wisc.edu writes:
>In article <3293A2DD.6BCF at pt.cyanamid.com>,
> "S. Silverman" <silvermans at pt.cyanamid.com> wrote:
>->Samuel C. Blackman wrote:
>->>
>->> My labmates and I, obviously with too much time on our hands, were
>->> wondering why we put our 32P-labelled autorads in the freezer at -80C.
//////////////
>-> The absorption and emission properties of the material in
>->intensifying screens depend on temperature. The screens are more
>->efficient at low temp. It will take someone with more familiarity with
>->molecular orbital theory than me to give you a more precice reason.
>
>I don't think so. Intensifying screens emit light upon absorption of
>high-energy particles. Light excites silver on film. Freezer is to
>increase sensitivity of latter.
Please see my post under "Re: Re: Why freeze autorads".
It is the screen & not the film which responds; this is experimentally demonstrated.
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