Protein binding to eppendorf/cuvette walls.

Bernard Heymann heymann at ubaclu.unibas.ch
Mon Nov 25 13:19:11 EST 1996


Many commercial enzymes sold as solutions include either BSA or glycerol
to prevent adsorption to the walls of tubes.  Also, ethylene glycol
(5-10%) is used in the buffer for thrombin to keep it from sticking to
tube walls.  Without the EG, you just don't get any thrombin activity.

I hope this helps

-- 
Bernard Heymann
M. E. Müller Institut, Biozentrum
Universität Basel
CH-4056 Basel
Switzerland
heymann at ubaclu.unibas.ch



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