Elution of protein from SDS-Page slice for immunization
Peter B. Kos
kp at everx.szbk.u-szeged.hu
Tue Nov 26 01:17:08 EST 1996
Ralf Spenneberg wrote:
> Hi there,
> i would like to purify an antigen on SDS-PAA-Page prior immunization of
> mice. Now i am looking for the most appropiate method or device which
> allows me to do that nice
> and easy !?!
> Is there anybody who can supply me with some tips about passive diffusion,
> chemical or electroelution?
> Any help is greatly appreciated.
> Ralf Spenneberg
> Klinische Forschergruppe für Endothelzellbiologie
> Universitaet Muenster
> Von Esmarchstr 56 48149 Muenster Germany
The best way could be the electroelution of the band in a dialysis tube.
The passive diffusion is sometimes used in vivo, which is the injection
of the (deep-frozen grinded gel slice)-suspension itself, but it is quite
difficult in mice and is most often used in bigger animals like rabbits.
However, this is the way to make large surface of the gel slice if you
decide in behalf of in vitro passive diffusion.
Peter B. Kos, PhD
email: kp at everx.szbk.u-szeged.hu
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