SDS PAGE DIGESTION

Dr E. Buxbaum EB15 at le.ac.uk
Tue Nov 26 11:02:17 EST 1996


Philippe Lefebvre <plefebvre at pop.univ-lille2.fr> wrote:

>Is there someone out there knowing a reliable method to digest SDS-PAGE
>slices?  They will be used further for scintillation counting.


The least messy way is to use crosslinkers with S-S bridges instead of 
bisacrylamid. These gels dissolve upon incubation with reducing agents. 
Pierce for example have them in their catalogue.







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