Streaking on nitrocellulose and PVDF membranes

[Dr E. Buxbaum]

a8803349 at unet.univie.ac.at (Martin Offterdinger) wrote:
>How old is the acrylamide solution you use. AA should be prepared
>every two month freshly because AA hydrolyses to acrylic acid and than
>your gels may exhibit uneven polymerisation causing smearing.

Alternatively, keep a few beads of anion exchanger in the bottle to 
absorb any acrylic acid that may form.