How to show protein interaction?

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Thu Nov 28 04:05:45 EST 1996


Thorsten Schmidt (Thorsten.Schmidt at rz.ruhr-uni-bochum.de) wrote:
> I have a protein and I just want to test if it forms dimers, trimers etc.?
> (The protein was purified by HisTag-Chromatographie and is in solution
> (20mM Tris, 0,5 M NaCl, 1 M Imidazol))
> What is the simplest way to do this?

I'd try analytical gel filtration if you have the equipment (i.e. an
FPLC/HPLC). Alternatively, you may try analytical ultracentrifugation
(I have absolutely no experience with this) or maybe non-denaturing
gel electrophoresis.

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP3 */
/* "Science is the game we play with God to find out what His rules are."  */



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