SDS PAGE DIGESTION

Dr E. Buxbaum EB15 at le.ac.uk
Fri Nov 29 11:05:36 EST 1996


Philippe Lefebvre <plefebvre at pop.univ-lille2.fr> wrote:
>> The least messy way is to use crosslinkers with S-S bridges instead of
>> bisacrylamid. These gels dissolve upon incubation with reducing agents.
>> Pierce for example have them in their catalogue.
>
>This is a bright idea, but SDS-PAGE are usually run with beta
>mercaptoethanol
>Dr Phil Good

That doesn't matter. A small volume of sample with a low concentration of 
bME at low temperature won't harm the gel. 




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