How to do NATIVE PAGE?

[Thorsten Schmidt]

Dear Reader!

I want to separate my target protein from other proteins in low
concentration
via native PAGE (poly acrylamide gel electrophoresis).
What do I have to think about when planning the experiment?
Where are the differences between native PAGE and SDS-PAGE?

I heart that one has to calculate the isoelectric point.
But how can I do this with a 300 aa-protein? (the sequence is known)

Thank you for your answer!

Thorsten Schmidt