Help! Dimerisation.

Mr. G. Morley gmorley at hgmp.mrc.ac.uk
Thu Sep 5 10:04:37 EST 1996


I am trying to design an experiment to measure
the extent of dimerisation between two homodimers.
I was wondering if anyone could recommend a 
crosslinker that can be used In Vitro. 
Ideally the cross-linker would be membrane
permiable and can be used on Rat-2 fibroblasts
(so not too toxic).If anyone uses or knows of a good
one..or has a methodology or practical tips I would be
most obligued. Currently I am thinking of DMP or DMS from 
Pierce..if anyone knows about any drawbacks about these 
two I would, also, be most obligued.
Thank you in advance...
																								Gary Morley
																								gmorley at rpms.ac.uk




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