help, buffer solution

Luis Lopes da Fonseca llfonseca at
Fri Sep 6 01:06:59 EST 1996

I have been asked the following questions, that i am not able to

How would you select a buffer solution, if you wanted to:

a) purify an enzime
b) characterize kineticly an enzime associated 
   with the glicolitic pathway
c) determine the enzimatic activity of a NAD dependent dehydrogenase

and why (justify)?

I thank all the help.

llfonseca at
Lisbon, Portugal

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