Help! Dimerisation.

Technical Assistance TechAssist at pierce.geis.com
Fri Sep 6 13:18:04 EST 1996


Mr. G. Morley wrote:
> 
> I am trying to design an experiment to measure
> 
> the extent of dimerisation between two homodimers.
> 
> I was wondering if anyone could recommend a
> 
> crosslinker that can be used In Vitro.
> 
> Ideally the cross-linker would be membrane
> 
> permiable and can be used on Rat-2 fibroblasts
> 
> (so not too toxic).If anyone uses or knows of a good
> 
> one..or has a methodology or practical tips I would be
> 
> most obligued. Currently I am thinking of DMP or DMS from
> 
> Pierce..if anyone knows about any drawbacks about these
> 
> two I would, also, be most obligued.
> 
> Thank you in advance...
> 
> 
> 
> 
Both the DMS and DMP would work in this application, however the imidoesters, 
in general, have a tendency to "leak". I suggest using NHS esters in their place. 
The NHS esters will not "leak" and they are available in both membrane 
permeable and membrane non-permeable forms. 
Tom Brotcke/Pierce Tech.



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