Request info on semi-dry blotters :)

[Cornelius Krasel]

BroweC (browec at aol.com) wrote:
> How do the
> various companies compare (Bio-rad, Hoeffer, etc.)?

We happen to have a BioRad model, and I am satisfied with it.

> How does semi-dry
> compare with standard blotting?

Some people claim that tank blotting is more sensitive. IMO a large
advantage of semi-dry blotting is that you need only a fraction of
the buffer, since preparing of buffers is not one of my favourites :-)

> Is stacking the gel sandwiches on top of
> one another to do multiple blots at once a problem?

Never tried this -- actually I never thought of this :-)

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP3 */
/* "Science is the game we play with God to find out what His rules are."  */