Morello Jean-Pierre tricky ligation

bg32 Beatrice_A_GRABOWSKI at UMAIL.UMD.EDU
Thu Sep 12 09:16:14 EST 1996


Hi.  You may be losing your insert in the Gene Clean and in the EtOH
extraction if the concentration is too low.  Start with what you think is an
impossibly large amount of insert.  If you gel purify, use agarase and
Amicon filtration to recover your DNA.  B. Grabowski



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