purifying translation products

Thomas Weber tomweber at bway.net
Thu Sep 12 08:04:02 EST 1996


Hi there,
are you using RNAse treated Retic lysate ? If not try this first. My 
guess would be that your biotinylated Lysine is incorporated into 
other Proteins which are in vitro translated from endogenous mRNAs.

regards

Thomas



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