Visualising Large Proteins

Virginia Urquidi vurquidi at molbiol.ox.ac.uk
Fri Sep 13 11:33:25 EST 1996


In Article <jd-1209961036110001 at lacto.path.cam.ac.uk>, jd at mesher.cam.ac.uk
(J Doog) wrote:
>Dear Reader
>
>I am trying to study the expression of a protein whose mass is
>approximately 290 kDa. As it is so large, visualisation on an
>SDS-polyacrylamide gel followed by Western blotting has proved difficult.

You could try  electrophoresis in agarose gels [horizontal or vertical] with
MetaPhor XR agarose [FMC Bioproducts]. They claim it can resolve proteins up
to 600 kDa. Coomassie Blue staining and electroblotting seem to be
compatible with it. No, I haven't tried it myself yet but I intend to, to
resolve multimers of a 65 kDa protein.

FMC Bioproducts
USA-Maine/Phone 207-594-3495; Fax:207-594-3491
Europe-Denmark/Phone: 45 43 731122; Fax: 45 43 735692

Best of luck

No affiliation et. etc.

V. Urquidi
Nuffield Dept. Clinical Biochemistry
Oxford University
vurquidi at molbiol.ox.ac.uk




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