Protein complex stability in SDS laemli buffer

Inger Natvig inger.natvig at
Mon Sep 16 08:12:19 EST 1996

We are currently working to characterize a receptor-ligand complex
belived to be non covalently associated.However,when run on SDS-PAGE 
according to Laemlis procedure this complex seems to be covalently 
associated .Which involves incubation in 2% SDS sample buffer at 37¤C 
for 1 hour, before loading on 1% SDS-PAGE.Does anyone has comments to 
protein complex stability under. I should also mention that boiling in 
SDS buffer result in protein aggregation. 

I look forwardto any comments

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