Protein complex stability in SDS laemli buffer

lpdanon at weizmann.weizmann.ac.il lpdanon at weizmann.weizmann.ac.il
Wed Sep 18 07:40:18 EST 1996


In article <51jjnj$3t0 at ratatosk.uio.no>, inger.natvig at rh.uio.no (Inger
Natvig) wrote:

> We are currently working to characterize a receptor-ligand complex
> belived to be non covalently associated.However,when run on SDS-PAGE 
> according to Laemlis procedure this complex seems to be covalently 
> associated .Which involves incubation in 2% SDS sample buffer at 37¤C 
> for 1 hour, before loading on 1% SDS-PAGE.Does anyone has comments to 
> protein complex stability under. I should also mention that boiling in 
> SDS buffer result in protein aggregation. 
> 
> I look forwardto any comments
> Inger 

Did you include a reducing agent, such as 2-mercaptoethanol or preferably
DTT,  in your sample buffer?



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