Column Choice

Dima Klenchin klenchin at facstaff.wisc.edu
Thu Sep 19 11:25:58 EST 1996


In article <51phdu$5mh at winx03.informatik.uni-wuerzburg.de>,
   krasel at wpxx02.toxi.uni-wuerzburg.de (Cornelius Krasel) wrote:
->Dima Klenchin (klenchin at facstaff.wisc.edu) wrote:
->> In article <51i1cl$1r6 at agate.berkeley.edu>, lhom at nature.berkeley.edu 
(Louis Hom) wrote:
->> #I need to run a sizing column to see if my ca. 30kD protein is 
associated
->> #with other proteins.  Can folks recommend places to look where I can 
find
->> #out what the best matrix and respective column sizes would be?  (I 
might be
->> #running this by gravity, maaaybe by FPLC, depending on things.) 
->> 
->> Well, if FPLC, then only Superose 12 makes choice. 
->
->Um, why not using Superdex 75?

Does not resolve well 30 K from 60 K, for example.
(I'm talking about "Prep" version, cause normal one comes
in too bloody expensive columns only).

- Dima





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