krasel at wpxx02.toxi.uni-wuerzburg.de
Thu Sep 19 07:29:40 EST 1996
Marcus, Dr J. (J.Marcus at botany.uq.edu.au) wrote:
> We are interested in detecting expressed proteins in
> plant extracts and need to be able to detect 0.1% or less
> of a total protein extract. On a small gel that would come
> out to about 50ng if 50micrograms were loaded.
This mostly depends on the quality of your first antibody. ECL works
fine for us; we haven't tried any other system. There have been
postings on how to make the ECL reagents yourself if your budget
is tight; search at http://net.bio.net/ for those.
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany email: phak004 at rzbox.uni-wuerzburg.de SP3 */
/* "Science is the game we play with God to find out what His rules are." */
More information about the Proteins