Western blotting

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Thu Sep 19 07:29:40 EST 1996


Marcus, Dr J. (J.Marcus at botany.uq.edu.au) wrote:
> We are interested in detecting expressed proteins in 
> plant extracts and need to be able to detect 0.1% or less 
> of a total protein extract.  On a small gel that would come 
> out to about 50ng if 50micrograms were loaded.

This mostly depends on the quality of your first antibody. ECL works
fine for us; we haven't tried any other system. There have been
postings on how to make the ECL reagents yourself if your budget
is tight; search at http://net.bio.net/ for those.

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP3 */
/* "Science is the game we play with God to find out what His rules are."  */



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