Isolation protocol for inclusion bodies

Alexander P Rostovtsev rosto001 at maroon.tc.umn.edu
Fri Sep 20 15:58:35 EST 1996


N.Schroten at med.ruu.nl (Niek Schroten) writes:

>Hi,

>I'm trying to isolate a 103 kD protein in BL21(DE3) in inclusion
>bodies. Is anyone familiair with isolation of inclusion bodies in
>combination with a protein of this weight ? Does anyone have a useful
>protocol for inclusionbody isolation ?
>Thanking you in advance for your help,

>Niek Schroten.

Biochemistry (1995)34:1858-1866
ibid (1995)34:5773-5794



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