Isolation protocol for inclusion bodies

[Alexander P Rostovtsev]

N.Schroten at med.ruu.nl (Niek Schroten) writes:

>Hi,

>I'm trying to isolate a 103 kD protein in BL21(DE3) in inclusion
>bodies. Is anyone familiair with isolation of inclusion bodies in
>combination with a protein of this weight ? Does anyone have a useful
>protocol for inclusionbody isolation ?
>Thanking you in advance for your help,

>Niek Schroten.

Biochemistry (1995)34:1858-1866
ibid (1995)34:5773-5794