Isolation protocol for inclusion bodies

Guy Oshiro Guy.Oshiro at
Mon Sep 23 13:18:09 EST 1996

Have you tried adding Sarkosyl to your lysis buffer.  It is a great
detergent to get get most proteins into the soluble fraction.  There
are a couple of papers on the subject but I don't have them handy.

Guy Oshiro
EMAIL: Guy.Oshiro at

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