SDS-page

David Aldridge aldridge at mbcmr.unimelb.edu.au
Mon Sep 30 19:51:42 EST 1996


This can happen if your protein sample is not completely reduced prior
loading on the gel. Try using 100mM FRESH DTT or somethimg similar
immediately prior to loading.

> Jussi Kankare wrote:
> > 
> > Dear Netters,
> > 
> > I'm trying to crystallize a protein and would therefore like
> > to have my sample as homogeneous as possible. However when the
> > purity of the sample is checked with SDS-page the gel shows a
> > major and small minor band just beneath the major one. I have
> > not purified the sample myself but the persons who have done
> > the work claim that the sample is pure but the protein just has
> > the property that it shows two bands in SDS-page ?!? I would naturally
> > think that this means that the sample is NOT homogeneous. What could
> > cause this sort behaviour of a protein or is there simply a need for
> > repurifying the protein.
> > 
> > Best Regards,
> > 
> > Jussi
> > --
>



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