Help me! AIR BUBBLES trapped in HPLC column...
lin at formosa.g1.ntu.edu.tw
Tue Apr 1 04:54:10 EST 1997
Rainer Hofmann wrote:
newera at plaza.snu.ac.kr () wrote:
>Some or probably quite many air bubbles seem to be trapped in my
HPLC column: 18C reverse phase column, pore size 7um.
>Could you give me any suggestions to remedy this problem as quickly
>I am much disappointed not to be able to proceed my experiments any
I guess the problem is poor degassing of solvents. At pressure
in a hplc column there aren't any free gas bubbles. But when
drops at the column's outlet gas is set free again and bubbles
You should use Helium to purge solvents and to keep them under a
Helium atmosphere. Online degassing systems are also available.
rhofmann at freiburg.netsurf.de
Kalkackerweg 20 fax: +49 761
79111 Freiburg phone: +49 761
You can degas solvents under sonicator and use air pump to remove air
bubles in solvent.
Try it, it works to me many years!!
Institute of Zoology,
National Taiwan Univ.
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