Help me! AIR BUBBLES trapped in HPLC column...

[Dr E. Buxbaum]

LIn Chung-Yen <lin at formosa.g1.ntu.edu.tw> wrote:

> Hi:
>You can degas solvents under sonicator and use air pump to remove air
>bubles in solvent.
>Try it, it works to me many years!!

Just remember to do this with the pure chemicals, before you mix them 
into the solvent mixtures commonly used in HPLC. If you degass the 
mixtures, their composition may change due to evaporation.