mdbci001 at CMU.CHIANGMAI.AC.TH
Mon Dec 1 10:14:50 EST 1997
How about periodate treatment? I have just read one protocol that they
used sodium periodate (10mM) in PBS for 15 minutes. They should remove
neary all of carbohydrate.
Prachya, CNX, Thailand.
On Mon, 1 Dec 1997, Roger Murphy wrote:
> Hi fellow protein chemists!
> Does anyone have a quick, simple recipe for removing N- and/or O-linked
> glycans from monoclonal antibodies? We want to determine the protein MW with
> and without post-translational modification of the glycan chain.
> Roger Murphy
> Roger Murphy, Ph.D.
> Biological Production Facility
> Ludwig Institute for Cancer Research
> Austin & Repatriation Medical Centre
> Studley Road,
> Heidelberg, Vic. 3084
> Tel 61-3-94965463
> Fax 61-3-94965436
> Email murphy_r at licre.ludwig.edu.au
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