de-glycosylation

Prachya Kongtawelert mdbci001 at CMU.CHIANGMAI.AC.TH
Mon Dec 1 10:14:50 EST 1997


Dear Roger,
How about periodate treatment? I have just read one protocol that they 
used sodium periodate (10mM) in PBS for 15 minutes. They should remove 
neary all of carbohydrate.
Prachya, CNX, Thailand.

On Mon, 1 Dec 1997, Roger Murphy wrote:

> Hi fellow protein chemists!
> 
> Does anyone have a quick, simple recipe for removing N- and/or O-linked 
> glycans from monoclonal antibodies?  We want to determine the protein MW with 
> and without post-translational modification of the glycan chain.
> 
> Thanks,
> 
> Roger Murphy
> 
> 
> 
> Roger Murphy, Ph.D.
> Biological Production Facility
> Ludwig Institute for Cancer Research
> Austin & Repatriation Medical Centre
> Studley Road,
> Heidelberg,  Vic. 3084
> Australia.
> 
> Tel  61-3-94965463
> Fax  61-3-94965436
> Email murphy_r at licre.ludwig.edu.au
> 
> 



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