Protein Labeling? Casein Kinase?

Thomas J. Albert tjalbert at students.wisc.edu
Sun Feb 2 00:56:07 EST 1997


I have used Casein Kinase II to lable a protein from cytosol, and then
was able to immunoprecipitate it, producing a nicely labled band. My
protein has four CKII consensus sites (minimum D/E-X-X-S/T), with
several other acidic residues in close proximity. Check if your protein
has any CKII sites, and if so, CKII is commercially available (I use
B-Mannhein), and should work well. I have no experience with CKI. E-mail
if you need CKII rnx conditions.

Tom



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