CD analysis of beta-sheet

Ronald Smulders ronsmul at
Tue Jan 7 10:36:11 EST 1997

Hi CD experts

I'm working on a typical beta-sheet protein. So the CD spectrum displays a
minimum at about 218 nm and the curve crosses the x-axis at about 200 nm. 
Now, when we analyze the protein after a period of heat exposure we see
an increase of the amplitude: so the spectrum does not shift but only the 
ellipticity increases. Does anyone know how to interprete this result?  Does
this result indicate that the amount of random coil increases upon heating?

Thanks in advance for your comments

Ronald Smulders, The University of Nijmegen

More information about the Proteins mailing list