Wenjin Yu wyu at
Fri Jan 10 07:38:11 EST 1997

Dear colleagues,

	We've isolated and cDNA encoding a protein which should have a 
weight of 25 kD dased on computer program calculation.  However, after we 
did Western blot using antibodies specific to this protein we got a 33kD 
band on SDS-PAGE (under reduced conditions).  Although this protein is a 
glycoprotein, it shouldn't change so much since only one glyco-site was 
found.  Does any one can explain this for me?  Any suggestions are highly 

Please respond by e-mail.

Wenjin Yu
     ||   Wen-Jin Yu             *  E-Mail: wyu at          || 
     ||   Dept. of Botany        *  Tel.(home): (519)836-2868        ||  
     ||   University of Guelph   *  Tel.(work): (519)824-4120, x4779 ||
     ||   Guelph, Ont. Canada    *  Fax:        (519)767-1991        ||
     ||   N1G  2W1               *  URL: ||      

More information about the Proteins mailing list