Affi-Chromat. with antibodys????

jaensch at mpimg-berlin-dahlem.mpg.de jaensch at mpimg-berlin-dahlem.mpg.de
Fri Jan 10 11:38:01 EST 1997


I would like to purify a protein complex with the help of affinity
chromatography. Therefore, I coupled an antibody, which recognizes one subunit
to CNBR-Sepharose (Pharmacia).
I have the following questions in this regard:

1. Is it resonable to couple antibodys to CNBR-Sepharose?

2. Which buffers are recommended for the affinity binding and for the elution

Thank you for helping me!!!!!

Lothar Jaensch, Universitaet Hannover
of the protein complex? (The antibodys should not be damaged)




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