How can I extract a protein from an SDS-PAGE

Ben Herbert bherbert at rna.bio.mq.edu.au
Thu Jan 16 11:21:15 EST 1997


Hi Jim

I guess you are talking about coomassie stained SDS gels??  If that is 
the case then you can extract the protein by excising the band and 
placing it into an eppendorf tube with about 200uL of 100mM Na acetate, 
0.1% SDS and 10mM DTT.  Shake for 24hours at 37 deg C and most of the 
protein will elute from the gel.  The protein can be recovered in this 
way for amino acid analysis, sequencing or whatever.



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