duncant at vax.cs.hscsyr.edu
Fri Jan 17 11:29:07 EST 1997
Richard P Grant wrote:
> We have a domain that is a bit capricious - it tends to precipitate
> and is reasonably unstable in solution. We'd like to stabilize it and
> I thought of introducing a judicious S-S by sdm. Does anyone know if
> this has been done, or is there a reference that I might look up?
> Richard P. Grant MA DPhil University of Oxford
> Nuffield Department Obstetrics and Gynaecology FFPGP
> http://users.ox.ac.uk/~lady0266 http://www.molbiol.ox.ac.uk/~rgrant
> --------------# The Internet is _full_. Please go away #--------------
One good article I have is
"Probing the Determinants of Disulfide Stability in
Native Pancreatic Trypsin Inhibitor". Goldenberg et al., 1993.
Biochemistry _32_, 2835-44.
It also references a number of articles that introduced disulfides in
attempts to stabilize proteins. Good luck.
Thomas M. Duncan
Dept. Biochemistry & Molecular Biology
SUNY Health Science Center
750 E Adams St, Syracuse, NY 13210
Email: duncant at vax.cs.hscsyr.edu
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