APS & TEMED in acid gels

Louis Hom lhom at nature.berkeley.edu
Mon Jan 20 13:11:50 EST 1997

So I'm running these HOAc-urea polyacrylamide gels (15% resolving, 4%
stack) and each part takes >1hr to polymerize at the concentrations of APS
and TEMED that I currently use.  Will I mess things up by increasing the
APS & TEMED concentrations so that polymerizatin occurs more on the order
of 30 minutes instead?  I won't be transferring or eluting the proteins
from the gel for anything.  
Lou Hom >K '93	
lhom at nature.berkeley.edu  

More information about the Proteins mailing list