Can I prepare SDS-PAGE gel mixes in advance?
Marc.Saric at rz.ruhr-uni-bochum.de
Tue Jan 21 17:21:20 EST 1997
On 12 Jan 1997 13:40:33 GMT, "Thorsten Schmidt"
<Thorsten.Schmidt at rz.ruhr-uni-bochum.de> wrote:
>I make many Western blots and SDS-PAGE-gels.
Have done some too :-))
>For each gel, I have to mix
>- Acrylamid-Mix (29 % acrylamid / 0,8 % bis-aa)
>- 10 % SDS
>- 10 % APS
>to pour a 3% and a 10% gel.
>Is there anything to be said against preparing a 3 % and a 10 % gel-mix
>by mixing the componets (except for APS & TEMED) in advance?
We made a new mixture every morning -that´s what is the usual way I
think (a bit boring to do the same work every day -I know)
>It would minimize time by just adding TEMED & APS to a aliquot!
>Or isn´t it good to store such a mix for a longer time?
This should depend on what is a "longer time". Looking at the
chemicals used for the gel, it should work, maybe you can store it
cool and dark, that´s it.
But I don´t know exactly.
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