MALDI-TOF is suitable to identify whether a protein is a dimer or a trimer?

Lee, Ji Hyun newera at plaza.snu.ac.kr
Fri Jan 24 00:07:50 EST 1997


MALDI-TOF analysis is suitable to identifying whether a protein is a
homodimer or a homotrimer?

I have a small DNA-binding protein(6,100 dalton) quite well purified. 
Through size exclusion HPLC gel chromatography, I found its effective
molecular weight of the protein in the absence of its binding DNA
sequence is about 19,000 dalton, close to homotrimer. 
But I am not sure that it is a trimer based on only the result becuase I
didn't ever hear about homotrimer DNA binding protein; so I need some
additional experiments confirming this fact.

Thank you for your time and expertise,

Lee, Ji Hyun



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