MALDI-TOF is suitable to identify whether a protein is a dimer or a trimer?
rlevine at nih.gov
Tue Jan 28 08:13:17 EST 1997
As the responses have pointed out, MALDI-TOF conditions always (my
experience) lead to dissociation of non-covalent subunits. However, it
seems to me that a simple solution is to add a cross-linking reagent to
your sample, locking it in the correct quaternary structure. After
adding a quenching agent to take up the excess cross-linker, just run
Has anyone tried this?
More information about the Proteins