Pharmacia FPLC UV

Ian McFarlane I.McFarlane at
Thu Jul 3 13:27:07 EST 1997


If I have a peak of protein (say BSA) on a gel exclusion column which
gives me 50% of full scale deflection at 1AU=FSD _approximately_ how much
protein is that? I know you need to measure area under the curve but can
anyone give me a ballpark figure?

Thanks in advance,

Ian Mc

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