E. coli strain BL21

Dr. Peter Gegenheimer PGegen at kuhub.cc.ukans.edu
Mon Jul 7 23:33:08 EST 1997

In <5pgtbd$3gt$1 at news.usf.edu>, "Wen-Ming Yang" <yangwm at moffitt.usf.edu> writes:
>In article <mbzrl-240697094355 at pmbfjd0.nottingham.ac.uk>,
>mbzrl at mbn1.biochem.nottingham.ac.uk (Rob) wrote:
> I am thinking of using E. coli strain BL21 to express a recombinant
>protein but I have heard that it carries certain health and safety risks.
>Does anyone have experience in using this strain or know of any special
>handling procedures or containment levels required to grow this strain.

No special precautions are required for use of E. coli BL21(DE3) except those associated with the gene being expressed. For containment purposes, remember that BL21 is an E. coli B strain, not a K12 strain. 

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