Acid native-PAGE buffer(s)

steve dunnsm at bbsrc.ac.uk
Wed Jul 9 08:40:38 EST 1997


Hello All,
I'm planning on performing a native-PAGE expt. on a couple of proteins
with fairly high predicted pI values (approx. 8.8-9.0) and so I'm looking
for an acid buffer system that operates at around pH 5-6 (I'd rather not
go more acid than this). Does anybody have tried and tested recipes for
a discontinuos system in this pH range?
Many thanks for any info/advice.
Steve
dunnsm at bbsrc.ac.uk



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