Pharmacia FPLC UV

Dima Klenchin klenchin at facstaff.wisc.edu
Wed Jul 9 16:57:35 EST 1997


In article <I.McFarlane-0307971827070001 at mac052034.lif.icnet.uk>, I.McFarlane at icrf.icnet.uk (Ian McFarlane) wrote:
:Hi,
:
:If I have a peak of protein (say BSA) on a gel exclusion column which
:gives me 50% of full scale deflection at 1AU=FSD _approximately_ how much
:protein is that? I know you need to measure area under the curve but can
:anyone give me a ballpark figure?

Yuo can very roughly consider that to be ~ 0.5 mg/ml. As to how much 
protein, this obviously depends on volume of your peak.

- Dima





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