Problem GST-Fusion Protein in Ecoli

Luc CAMOIN luc.camoin at cochin.inserm.fr
Fri Jul 11 05:09:06 EST 1997


Hi,

I expressed a GST-fusion protein in Ecoli. The theoretical molecular weight
is 43 kDa. Western blot with anti-GST antibodies showed a band at 66 kDa.
Purification on Glutathione-sepharose-4B concentrate an unique band at 34
kDa. This 34 kDa band is not recognize with anti-GST antibodies.

I need some advice to explain these results. Is it known that a Ecoli
protein bind strongly to Glutathione affinity column? This 34 kDa is it a
protein or fragment related to my recombinant fusion protein?

I'am not familiar with these techniques so all advices will be welcome.

Thank you very much

Luc CAMOIN




Institut         _/_/_/_/  _/_/_/_/  _/_/_/_/  _/_/    _/       Luc CAMOIN
Cochin            _/      _/        _/        _/ _/ _/_/       CNRS UPR 415
de               _/      _/        _/  _/_/  _/   _/ _/      22 rue Mechain
Genetique       _/      _/        _/    _/  _/      _/   75014 Paris France
Moleculaire  _/_/_/_/  _/_/_/_/  _/_/_/_/  _/      _/ Tel:+33 1 40 51 64 98





More information about the Proteins mailing list