Help! Protein refolding problem

Alexander Schramm bmb030 at sp2.power.uni-essen.de
Wed Jul 9 07:36:23 EST 1997



Poi Ming Jye <poi.2 at postbox.acs.ohio-state.edu> schrieb im Beitrag
<poi.2.10.33C2C250 at postbox.acs.ohio-state.edu>...
> Hi,
> 	I have a problem in refolding my protein.  Well, the major problem is my

> protein has 7 disulfide bonds.  Therefore, in the process of refolding, I

> often lose a lot of protein due to many steps of refolding and misfolding
too. 
If you want to express the protein in E.coli you may add a leader peptide
to your protein, so that it is transported to the periplasm. This is the
compartment, where S-S-bridges will be correctly formed.
Alex
alexander.schramm at uni-essen.de




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