DAB as a Peroxidase Substrate

Thorsten Schmidt Thorsten.Schmidt at rz.ruhr-uni-bochum.de
Sun Jul 13 06:46:08 EST 1997


Dear Sir or Madam!

I use the Vectastain ABC Elite Kit with the DAB Substrate Kit for
Peroxidase for Immunhistochemistry.

My Question is:
What is the exact chemical reaction, which is catalysed by the
peroxidase?
Or, in other words, what is the exact chemical structure of the
reddish brown staining product which the Peroxidase synthesizes
out of DAB and hydrogen peroxide?

Thank you very much in advance for your answer.

Thorsten Schmidt



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