Protein precipitation in HPLC buffer

Marcus, Dr J. J.Marcus at
Tue Jul 15 17:18:49 EST 1997

> Hi!
> I am trying to purify a protein using C18 RP-HPLC. Unfortunately it
> precipitates when sample mixed with A buffer (0.1% TFA in water).
> Is there another good buffer system (pH>6) for the protein separation on

You can try phosphate buffer (say 10-20 mM, pH 2.0, 4.4 and 
6.5). Silica is notorious for not liking Alkaline pHs so 
I would not go above pH 6.5.  


John Marcus            Marcus at (Dr J.Marcus)
Cooperative Research Centre for Tropical Plant Pathology
5th Level John Hines Building
University of Queensland
St. Lucia, QLD 4072

Fax: 61-7-3365-4771
Phone: 61-7-3365-4764

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