Protein precipitation in HPLC buffer

Marcus, Dr J. J.Marcus at botany.uq.edu.au
Tue Jul 15 17:18:49 EST 1997


> Hi!
> I am trying to purify a protein using C18 RP-HPLC. Unfortunately it
> precipitates when sample mixed with A buffer (0.1% TFA in water).
> Is there another good buffer system (pH>6) for the protein separation on
> 

You can try phosphate buffer (say 10-20 mM, pH 2.0, 4.4 and 
6.5). Silica is notorious for not liking Alkaline pHs so 
I would not go above pH 6.5.  

Regards,
John




_________________________________________________________
John Marcus            Marcus at tpp.uq.edu.au (Dr J.Marcus)
Cooperative Research Centre for Tropical Plant Pathology
5th Level John Hines Building
University of Queensland
St. Lucia, QLD 4072
AUSTRALIA

Fax: 61-7-3365-4771
Phone: 61-7-3365-4764



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