Protein precipitation in HPLC buffer

Gundars Goldsteins Gundars.Goldsteins at uku.fi
Tue Jul 15 02:25:37 EST 1997


Hi!
I am trying to purify a protein using C18 RP-HPLC. Unfortunately it
precipitates when sample mixed with A buffer (0.1% TFA in water).
Is there another good buffer system (pH>6) for the protein separation on

C18 ?
TIA,
Gundars






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