switching cations

Dirkson E. Bussiere-42T bussierd at tvsfrank
Wed Jul 16 18:34:28 EST 1997

I'm trying to swap the Zn2+ in an enzyme with other divalent
cations, but everytime I do it, the protein precipitates.  I
more or less dialyze the protein in 50mM Tris-HCL, pH 7.4,
with 1-10 mM of the cation2+; the protein is stable in
the Tris by itself.  Does anyone know of a way to stablize the
transition?  One caveat:  the enzyme is quite concentrated
(2.1 mg/ml), and I have thought of diluting it a bit.  But I
will have to reconcentrate it to 12 mg/ml, so this does not
seem to solve the problem.

Any ideas would be appreciated.


Dirk Bussiere                 bussierd at tvsfrank.pprd.abbott.com 
Abbott Laboratories             Office: (847)935-0916
Department 42T                  FAX:    (847)937-2625
100 Abbott Park Rd.
Abbott Park, IL   60064-3500

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