protein-protein interactions

Zophonias O. Jonsson zjons at
Wed Jul 16 15:39:07 EST 1997

In article <sarafian.869076206 at njmsa>, sarafian at njmsa.UMDNJ.EDU (Stefan G.
Sarafianos) wrote:

> Hi,
> Anybody has any references or any info about techniques for detecting 
> protein-protein interactions with colorimetric or fluoresence techniqes?
> I f the method could be applied for interactions of the protein 
> with itself -polymerization- that would be great.
> Thank you for your time,
> Stefan G. Sarafianos
> post-doc
> Center for Advanced Biothechnology and Medicine
> Rutgers university/UMDNJ
> Oiscataway, USA

Try looking up some of the work of the groups of Peter H. von Hippel or
Stephen J. Benkovic on the phage T4 DNA replication fork.  They have
studied the interactions of the gp45 clamp (which forms a trimer) with
itself and other proteins using fluorescence.

Looks like just what you are looking for


Zophonias O. Jonsson
Institut fur Veterinarbiochemie               Tel: (41-1)-257-54-75
Universitat Zurich-Irchel                     Fax: (41-1)-362-05-01
Winterthurerstrasse 190
CH-8057 Zurich

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